Figure 1. Basic principle of AIR™ technology to achieve large density multiplex arrays. A) Using a silicon dioxide substrate with thickness specific to an array of antibody probes, near-zero reflectance is achieved when illuminated with s-polarized light at a fixed wavelength and angle B) Upon antigen binding to unique antibodies arrayed on individual spots, concentration dependent signal is detected using a CCD camera. Further enhancement of signal and increased sensitivity can be achieved by adding secondary antibodies or any large target enhancers that result in increased mass C) Individual biosensors can be assembled into a 96-well format that can be further be automated in a detection system (ZIVA) for complete automation.
- Mace, C. R., Striemer, C. C., Miller, B. L., “A theoretical and experimental analysis of Arrayed Imaging Reflectometry as a sensitive proteomics technique” Anal. Chem., 78, 5578-5583 (2006).
- Carter, J. A., Mehta, S. D., Mungillo M.V., Striemer, C. C., Miller, B. L., “Analysis of inflammatory biomarkers by Arrayed Imaging Reflectometry” Biosensors and Bioelectronics, 26, 3944-3948 (2011).
- Bucukovski, J.; Carter, J. A.; Striemer, C. C.; Müllner, S.; Schulte-Pelkum, J.; Schulz-Knappe, P.; Miller, B. L. “Label-free microarray-based detection of autoantibodies in human serum” J. Immunol. Methods, 459, 44-49 (2018).
- Zhang, H.; Henry, C.; Anderson, C. S.; Nogales, A.; DeDiego, M. L.; Bucukovski, J.; Martinez-Sobrido, L.; Wilson, P. C.; Topham, D. J.; Miller, B. L. “Crowd on a chip: Label-free human monoclonal antibody arrays for serotyping influenza”, Analytical Chemistry, 90, 9583-9590 (2018)
- Bucukovski, J.; Latorre-Margalef, N.; Stallknecht, D. E.; Miller, B. L. “A multiplex label-free approach to avian influenza surveillance and serology”, PLOS ONE, 10, e0134484 (2015)